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KMID : 1189420130260040001
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2013 Volume.26 No. 4 p.1 ~ p.14
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The Effects of Syzygium aromaticum extract Spread on the Allergic Contact Dermatitis induced by DNCB
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Lee Kyung-Yeob
Kang Da-Hae
Kim Hee-Taek
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Abstract
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Objectives : The purpose of this study is to investigate effects of Syzygium aromaticum (SAE) spread on the allergic contact dermatitis caused by 2,4-dinitro-chlorobezene (DNCB).
Methods : Forty-two mice were divided into six groups ; normal, negative control (DNCB-treated), positive control (DNCB + 1% pimecrolimus), experimental group I, II and III. control and experimental groups were induced allergic contact dermatitis by DNCB. Experimental group I(DNCB + 0.2% SAE), II(DNCB + 1% SAE) and III(DNCB + 5% SAE) were spread SAE and positive control was spread the 1% pimecrolimus. In this study, effect of SAE on clinical aspects on the skin, histopathological change, the blood level of IgE, cytokines, histamine were investigated. In addition, effect of SAE on spleen CD4^+/CD8^+ T cell subset was investigated.
Results :
1. In experimental group I, II and III, erythemas and edema were more reduced than negative control.
2. In experimental group I, II and III inflammatory edema and the numbers of infiltrated inflammatory cells were more reduced than negative control.
3. In experimental group I, II and III, clinical skin score was more reduced than negative control.
4. In experimental group II and III, the thickness of skin was statistically significant reduced than negative control.
5. In experimental group II and III, histamine release was statistically significant reduced than negative control in dose-dependantly.
6. In experimental group II and III, cytokines (IL-1β, TNF-α, IL-4, IL-6, IL-10) were statistically significant reduced than negative control in dose-dependantly.
7. In experimental group I, II and III, the level of total IgE was statistically significant reduced than negative control in dose-dependantly.
8. In experimental group III, CD4^+ and CD8^+ T cells were statistically significant decreased similar to the positive control.
Conclusions : According to above experiments, Syzygium aromaticum(SAE) was effective on allergic contact dermatitis.
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KEYWORD
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Syzygium aromaticum(SAE), Allergic Contact Dermatitis, DNCB, Th1, Th2, histamine, Spread
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